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Fermentation

Fermentation is involved in “system analysis and innovation of bioprocesses”, process engineering, dynamics of microbial production, microbial engineering, metabolic engineering, modelling, conception, optimisation and monitoring of bioreactors. In the field of microbial productions and bioconversions, for eukaryotic and prokaryotic microorganisms and under industrial constraints, the research activity is oriented towards the study of microbial behaviour within bioprocesses using quantitative macro kinetics, metabolic and genomic methodologies. Quantitative analysis of the system concerns the reconciliation of the macro-, microscopic and molecular observation levels coupled with the establishment of stochiometric and kinetic mathematical models. This approach aims to identify determining properties for the implementation of optimisation strategies for bioprocesses.

The CBS expertise and skills will be oriented towards:

  • the principles of rational implementation of microorganisms under technico-economic constraints
  • the recommendations of high cell density technologies (fed-batch, membrane bioreactors)
  • the extractive fermentation methods (coupling bioreactor and extraction operation units and help to the basic microbiology)

These activities are generic for red, yellow and white biotechnologies. A high level of methodology necessary to obtain reliable results will be co-developed. The need of a tool box in term of process engineering, biomathematics, modeling/simulation and scaling up/down is a strong help for transfer technology, innovation and bioproduction of biomaterials for research or samples for industry. CBS will be a strong partner for developing microbial process for industry. Mastering the fermentation is a key opener for cooperative research and training engineers from industry. It opens also the possibility of scaling up on realistic basis through the cooperation with specialists of process control and applied mathematics.

 

Microarrays

The CBS also needs to further build up a microarray platform to identify new genes, discover their functions and expression profiles, detect variation and mutations for diagnosis and traceability, study enzymatic activity and monitor health, food, water quality and biosafety. Bioinformatics is primordialy needed for the interpretation and valorisation of the results produced by all bioprocesses. It is critical that information about the sample and biochip preparation and handling is discussed in order to help identifying the independent parameters in the experiment and to avoid unjustified estimates of statistical significance. Microarray data is difficult to exchange due to the lack of standardization in platform fabrication, assay protocols and analysis methods. This presents an interoperability problem in bioinformatics. Various grass-roots open-source projects are trying to ease the exchange and analysis of data produced with non-proprietary chips. Via BioProtech, the set up and further investigation of the development of a high throuput analysis microarray platform in the CBS is expected. It will be particularly supervised by the BioProtech European partners through organisation of theoretical and practical workshops, lab courses, training, conferences and visits of seniors to occasionally conduct the platform. BioProtech will strengthen the capacities of the CBS to decide which array platform(s) comply with the CBS’s scientific and financial requirements, to design and perform microarray studies and retrieve as much exploitable significant information as possible from the huge datasets of microarray experiments. The platform will be open to external user service locally including private laboratories of medical analysis. The expertise of the BioProtech European partners will be of great importance to acquire autonomy for all genomic research applied in the health and nutrition domains.

Genomics technologies including DNA microarrays and new generation sequencing (NGS) are largely used in different fields of biology (medicine, nutrition, safety etc..). They are used to improve clinical diagnosis and prognostic and monitoring tools (biomarkers), to verify contamination of biological preparation (vaccine, food, blood), to characterize pathogens or to improve selecting markers in agriculture….

The implementation of these technologies is often associated with different issues

1) the cost of the equipment

2) the choice of the technology

3) the choice of the best protocols to give answers to specific problematic

4) the formation of technical staff

5)  the bioinformatics and statistics tools.

All of these items are time-consuming and cost effective, and sometimes a wrong choice in term of technology or protocol could be dramatic for the research centre. The success of the technology implementation is correlated to the reduction of these bottlenecks.

Regarding these issues, the success of implementation of these technologies in the CBS needs to be supported by a structure (considered as a tutoring structure or test laboratory) with a great expertise in this field and equipped with the most recent instruments. In this context, the platform profilexpert is equipped with the most recent technologies (for example new generation sequencing roche 454 and Illumina/solexa) operated by skilled personnel.

The project will allow

1) CBS technicians and researchers to have access to the platform to check different protocols and technologies before implementation in CBS

2) Formation of engineer in bioinformatics

3) the sharing of already developed and checked protocols.

 

Biosecurity

The linkage between all these fields is the use of microorganisms, molecular biology, DNA and protein technology, however, there’s a lack of rigorous application of biosafety measures in the CBS. The CBS currently uses genetically modified organisms (“GMOs”) beyond containment in the laboratory setting (for example, under confined conditions in growth chambers, glasshouses, field trials and beyond). Although researchers take care and are absolutely sensitive to such concerns, the CBS is not equipped with Biosafety Level 2 or 3 laboratories necessary to protect the environment and the staff.  Moreover there are no regular courses in biosafety regulations organised in the CBS; which is particularly distressing since the CBS is host of a hundred of PhD and masters students (junior researchers). The BioProtech European partners have a long experience in tailoring biosafety training workshops that best meet the needs of recipient institutions and their staff. The BioProtech European partners will help the CBS to implement the best practices and initiate a specific training for the safe handling, monitoring and conformity to national and international regulations. In addition, with the support of BioProtech European partners, the CBS will implement a structure for GMO design practices and research protocols.

Considering the sensitive sector of biotechnology and in order to promote the use and the transfer of bioprocesses as well as the healthy use of bioproducts, measures should be drawn to promote biosafety guidelines in the CBS. In the future, no one is allowed to use or disseminate biological materials without taking into account the drastic measures of biosecurity. In fact, entering in collaborative projects with European partners firstly means the consideration of all measures of security. At the industrial level, this is not a simple necessity but one of the major factors for transfer of technology. Involvement of European specialized institutions as well as competencies is necessary for the implementation of the CBS guidelines for bioisecurity. This is concomitantly occurring with the drawing of national measures and policies related to biosecurity and OGM use and production. BioProtech program will :

  • Increase CBS capacities for the safe handling and monitoring of microorganisms, active biomolecules and GMOs, in conformity to national regulations and in line with European practice and acquisition of best practices and research protocols to result in less stringent regulatory measures.
  • Set-up of a GMO detection laboratory that conforms to national requirements, incorporating key strategic advances in the area of sampling and traceability in line with current European standards and procedures in this arena
  • Improve the acquisition of basic safety laboratory practices in line with the WHO rules on biosafety, local legislation, and in conformity with EU directives

The linkage between all these fields is the use of microorganisms, molecular biology, DNA and protein technology, however, there’s a lack of rigorous application of biosafety measures in the CBS. The CBS currently uses genetically modified organisms (“GMOs”) beyond containment in the laboratory setting (for example, under confined conditions in growth chambers, glasshouses, field trials and beyond). Although researchers take care and are absolutely sensitive to such concerns, the CBS is not equipped with Biosafety Level 2 or 3 laboratories necessary to protect the environment and the staff.  Moreover there are no regular courses in biosafety regulations organised in the CBS; which is particularly distressing since the CBS is host of a hundred of PhD and masters students (junior researchers). The BioProtech European partners have a long experience in tailoring biosafety training workshops that best meet the needs of recipient institutions and their staff. The BioProtech European partners will help the CBS to implement the best practices and initiate a specific training for the safe handling, monitoring and conformity to national and international regulations. In addition, with the support of BioProtech European partners, the CBS will implement a structure for GMO design practices and research protocols.

Considering the sensitive sector of biotechnology and in order to promote the use and the transfer of bioprocesses as well as the healthy use of bioproducts, measures should be drawn to promote biosafety guidelines in the CBS. In the future, no one is allowed to use or disseminate biological materials without taking into account the drastic measures of biosecurity. In fact, entering in collaborative projects with European partners firstly means the consideration of all measures of security. At the industrial level, this is not a simple necessity but one of the major factors for transfer of technology. Involvement of European specialized institutions as well as competencies is necessary for the implementation of the CBS guidelines for bioisecurity. This is concomitantly occurring with the drawing of national measures and policies related to biosecurity and OGM use and production. BioProtech program will :

  • Increase CBS capacities for the safe handling and monitoring of microorganisms, active biomolecules and GMOs, in conformity to national regulations and in line with European practice and acquisition of best practices and research protocols to result in less stringent regulatory measures.
  • Set-up of a GMO detection laboratory that conforms to national requirements, incorporating key strategic advances in the area of sampling and traceability in line with current European standards and procedures in this arena
  • Improve the acquisition of basic safety laboratory practices in line with the WHO rules on biosafety, local legislation, and in conformity with EU directives.

 

end faq

Last Updated on Wednesday, 04 July 2012 22:03
 
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